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KMID : 0960220020010020269
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Journal of the Korean Balance Society
2002 Volume.1 No. 2 p.269 ~ p.269
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Expression of Immediate-Early Gene Products by Occlusion of Anterior Cerebellar Artery in Vestibular Nuclear Complex of Sprague-Dalwey Rats
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Kim, Min Sun
Choi, Myoung Ae/Choi, Dong Ok/Cho, Byung Gon/Jin, Yuan Zhe/Kim, Jae Hyo/Kry, Davy
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Abstract
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The purposc of this study was to evaluate expression of immediate-early gene products, metabolic marker of neural excitation in neuronal cells, in the vestibular nuclear complex by occlusion of anterior cerebellar artery(AICA) in adult Sprague-Dalwey rats. After chloral hydrate anesthesia all animals were received unilateral occlusion of ACA by using microsurgical clamp for 30 min to induce temporally ischemia in brain stem and inner ear. Imniunohistochemical staining and image analysis for cFos, FosB, Krox-24, and JunB proteins were performed 2 hours after occlusion of AICA. There was high expression of cFos protein in bilateral medial medial and inferior vestibular nuclei 2 hours after unilateral occlusion of anterior cerebellar artery(AICA). But AICA occlusion induced minimal change in cFos expression in lateral and superior vestibular nuclei. Mild to moderate expression of FosB and Jun B protein was observed 2 hour ischemic injury in brain stem and. inner ear. A few neurons in ve 3tibular complex showed immunoreactivity for Krox-24 protein following AICA occlusion. Furthermore pretreatment of bilateral labyrinthectomy resulted` in significant reduction of imrnunoreactivity for cFos, FosB, and Jima proteins in medial and vesitbular expressions 2 hours after unilateral occlusion of AICA. These results uggest that ischemia of peripheral vestibular apparatus play a major role in expression of immediate-early gene products in medial and inferior vestibular nuclei of rats.
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